Archive for the ‘Testing’ Category

Science, Public Health Policy & the Law Has Accepted Dr. Sin Hang Lee’s Study of the Flaws of Non-Quantitative RT-PCT For SARS-CoV-2 Detection – Part 1

**UPDATE**

Go here for another excellent read on how for a 5% prevalence rate for COVID infections in a population, the 42% false positive discovery rate means that for every 50 true positives, there will be 36 false positives.  Ponder that for a moment.

COVID cases will be overstated by a factor of 72%

Due to unreliable of PCR tests:

There are no credible COVID-19 ‘vaccine’ trial data.

https://popularrationalism.substack.com/p/science-public-health-policy

Science, Public Health Policy & the Law has Accepted Dr. Sin Hang Lee’s Study of the Flaws of Non-Quantitative RT-PCR for SARS-CoV-2 Detection – Part 1

Sanger Sequencing Provides Definitive Evidence that RT-PCR Use with No Internal Control Applied to the Problem of Diagnosis of COVID-19 is Fatally Flawed

James Lyons-Weiler

When the German team (Cormen et al. (aka “Drosten Report”)) published primers capable of detecting synthetic oligonucleotide that matched parts of the SARS-CoV-2 genome sequence published from the first clinical sample from the first patient diagnosis of pneumonia associated with a novel coronavirus, there was hope that the virus might be controlled at least until healthcare facilities could be made ready using the training and preparations they conducted during the Ebola care of 2014.

The use of PCR to detect specific sequences is trivially non-controversial, if it is done in an expert manner. First, the primers are chosen computationally to match only the target species (or quasi-species, in the case of viruses). Second, multiple targets can be selected that help nail down the results in case one of the single. Finally, if the primers are nested or hemi-nested – that is if the targets sequences overlap, a local sequence can reliably be determined on a percentage of the samples as a direct gold-standard check to estimate both the true positive rate (the probability of detecting a virus that is truly present) and the false positive rate (the probability of detecting the target when it is truly not present).

I expected when CDC’s PCR test failed that there would likely be a variety of approaches that would be developed by commercial nucleic acid technology companies. I started advocating for private mass testing, with an emphasis on “private”, expecting that accurate testing would emerge.

I was astonished when I saw that the FDA had only requested that commercial suppliers provide data on the true positive rate, but no data on the false positive rate. Worse, I was astonished to learn, upon reading the documents being submitted to the FDA, that the test kit manufacturers were not including positive control sample material to allow the determination of cycle threshold (Ct) by which one makes the call for a given patient that the virus was present or absent. Cycle thresholds are the number of rounds of amplification necessary to reach a specific point in the exponential growth of the number of copies of target sequences. This must be done for each patient separately – even if the test is the same kit done in the same lab, on the same day, by the same technician – because the amount of starting material in each swab varies.

This was the first time I had ever seen any RT-PCR-based test NOT use a positive control sample. The lack of a positive control sample means that the assay was qualitative, not the robust and rigorous qRT-PCR (“q” stands for “quantitative”. For example, even the RT-PCR test for the Monkeypox virus uses a positive control sample.

Instead of using empirically derived Ct values per patient, generic Ct values were used. These were part of the kits, but they were not published. This was unusual. I asked medical freedom activists to request from their local health department what Ct values were being used to determine a diagnosis for a patient. Multiple people did, and the reply was the same: that information is proprietary. This was ridiculous; the exact Ct being used is not a top-secret part of a test, but is, instead, an essential aspect of checking the reliability of the results of an RT-PCR test.

Go to top link for a video on RT-PCR.

The absence of a positive control target sample was a big deal because, without any data on whether tests were hitting human sequences via off-target amplification, many people would be “diagnosed” with COVID-19 who were not infected. This would not only be disruptive – the resulting huge numbers of false positives could be used to justify police actions as was going on in China at the time.

Further, people who had other respiratory illnesses like influenza, respiratory syncytial virus, bacterial pneumonia, the common cold, or other coronaviruses might not be given appropriate medical care given the isolate-and-do-nothing approach to COVID-19. Many would die from severe pneumonia that could have been prevented via medical intervention, just as antibiotics for bacterial pneumonia.

Also, it was clear that many people who were sick but did not have COVID-19 would believe thereafter they were immune, and they might risk exposures that they otherwise might not risk.

In addition, those that did then become infected after their false positive COVID-19 test might then doubt their actual case of SARS-CoV-2 infection was worth testing for and they might fail to protect others who were at the highest risk of death from COVID-19 infection – the immunocompromised and the elderly.

So I wrote to the FDA with my concerns. Dr. Peter Marks wrote back with a terse “Thank you, we will take your concerns up with my team”.

At the same time, Dr. Sin Hang Lee independently saw the same issues and was motivated to develop a Sanger sequencing-based test. His approach circumvented the risk of false positives altogether by using primer pairs that targeted parts of the SARS-CoV-2 genome in an overlapping mapper. This way, he could tell if he truly had the sequence of interest in a sample, or if one of the primer pairs was failing due to a mutation in the primer site.

Dr Sin Hang Lee in his laboratory in Milford, CT.

Dr. Lee is an extremely capable and experienced scientist and is an expert in molecular diagnosis and diagnostic pathology. The fact that he independently came to the same conclusion as I did on the risks of using RT-PCR the way CDC, ultimately, FDA allowed under emergency use authorization gave me hope.

July 2020 WWDNYK’s Unbreaking Science: CDC PCR Test Wrong 1/4 of the Time

See top link for a video from about the time I contacted FDA (Nov 2020).

After writing various blog articles and doing podcasts attempting to alert the public and health departments to these very serious issues, I was invited to testify in a case in Pennsylvania wherein a restauranteur was sued by the Commonwealth for not following the public health dictates regarding her customers.

Originally, the testimony was meant to be written. So I provided the judge with the scientific literature on measured false positive rates of the RT-PCR tests. There were about four studies that provided data that demonstrated that the false positive rates varied from 11% (Basile et al.) to as high as 38% (the Duke Marines study).

The State Epidemiology submitted her testimony at the same time, without seeing mine. (I did not see hers, either). When I was given a copy of her testimony, I could see she knew nothing about the state of the science on the use of RT-PCR as allowed by CDC and PCR. She reported to the judge in her written testimony that RT-PCR tests for COVID-19 had – get this – ZERO false positives.

That’s when the judge, for reasons I will never understand, refused both sets of written testimony, and instead decided that only verbal testimony was going to be allowed. Of course, the State’s lawyer used the only tool they had – ad hominem attack – to try to discredit me. But even that didn’t change the fact that RT-PCR tests were visiting abuse on the public via false positives as outlined above.

A group of people became increasingly aware of the issues, and together we created NAATEC, the Nucleic Acid Assay Technology Evaluation Consortium – to collect public funds and fund the comparison of RT-PCR testing to Sanger sequencing.

I am happy to report that we thereby funded research by independent research scientist Dr. Sin Hang Lee to conduct the evaluation that FDA should have required by PCR test kit manufacturers.

The study underwent a single-blind peer review with two independent reviewers not involved in the study. The peer reviewers were scientists and experts in the field. After two rounds of feedback from the reviewers, the manuscript was accepted for publication and is with the editorial production team.

Prior to publication, I am providing the title, author, and Abstract.

Evidence-Based Evaluation of PCR Diagnostics for SARS-CoV-2 and the Omicron Variants by Sanger Sequencing

Dr. Sin Hang Lee

Abstract: Both SARS-CoV-2 and SARS-CoV-1 initially appeared in China and spread to other parts of the world. SARS-CoV-2 has generated a COVID-19 pandemic causing more than 6 million human deaths worldwide while the SARS outbreak quickly ended in six months with a global total of 774 reported deaths. One of the factors contributing to this stunning difference in the outcome between these two outbreaks is the inaccuracy of the RT-PCR tests for SARS-CoV-2, which generated a large number of false-negative and false-positive test results that have misled patient management and public health policymakers. This article presented Sanger sequencing evidence to show that the RT-PCR diagnostic protocol established in 2003 for SARS-CoV-1 can in fact detect SARS-CoV-2 accurately due to the well-known ability of the PCR to amplify similar, homologous sequences. Using nested RT-PCR followed by Sanger sequencing to retest 50 patient samples collected in January 2022 and sold as RT-qPCR positive reference confirmed that 21 (42%) were false-positive. Routine sequencing of the RT-PCR amplicons of the receptor-binding domain (RBD) and N-terminal domain (NTD ) of the Spike protein (S) gene is a tool to avoid false positives and to study the effects of amino acid mutations and multi-allelic SNPs in the circulating variants for investigation of their impacts on vaccine efficacies, therapeutics. and diagnostics.

The study was partially funded by IPAK via the NAATEC. To support research like this, visit http://ipaknowledge.org/

Watch for Part 2 at the end of the month – the details of the study will be explained.

_________________

**Comment**

Dr. Lee is an unsung hero who has been outspoken many times against medical injustice:

Category:

Activism, research, Testing, Viruses

Anaplasmosis & Stabbing Headache

https://danielcameronmd.com/anaplasmosis-leading-to-neurological-symptoms-of-trigeminal-neuralgia/

Anaplasmosis leading to neurological symptoms of trigeminal neuralgia

anaplasmosis-neurological-symptoms

Anaplasmosis, formerly called human granulocytic anaplasmosis (HGA) is a co-infection from a tick that typically causes acute disease. In their article, “Trigeminal Neuralgia As the Principal Manifestation of Anaplasmosis: A Case Report,” LeDonne and colleagues described human granulocytic anaplasmosis in an 80-year-old woman with neurological symptoms. [1]

By

According to the authors, the woman experienced a “sudden onset of severe, lancinating headache in the distribution of the fifth cranial nerve bilaterally.”¹ She had been treated for Lyme disease two months earlier following a tick bite and a rash on her torso. She had since been bitten by a non-engorged tick.

Her neurologic exam was felt to be consistent with a diagnosis of trigeminal neuralgia. Trigeminal neuralgia (TN) is a type of chronic pain that affects your face. It causes extreme, sudden burning or shock-like pain. It usually affects one side of the face.²

The patient’s mother had a history of trigeminal neuralgia. The doctors did not see any evidence of Lyme disease.

“However, anaplasmosis and ehrlichiosis can both develop over a shorter timeframe and without a noticeable rash, making these infections a more likely explanation of the patient’s signs and symptoms,” wrote the authors.

They added, “To confirm the suspected diagnosis, a tick-borne disease panel was ordered and was positive for Anaplasma phagocytophilum DNA by PCR.”

“Although severe headache is a common presenting symptom in patients with anaplasmosis, prior studies have not linked anaplasmosis and trigeminal neuralgia,” the authors explained.

“Our case suggests that anaplasmosis was the cause of our patient’s new-onset trigeminal neuralgia.”

The woman was treated with a 3-week course of doxycycline for Anaplasmosis and was prescribed gabapentin 300 for her trigeminal neuralgia.

She had marked improvement in her headaches. Her leukopenia, thrombocytopenia and abnormal hepatic enzymes returned to normal.

In their article, the authors reviewed the literature on Anaplasmosis, pointing out that the symptoms of Anaplasmosis are non-specific and may include fever, myalgia, and headache but no rash. In addition, patients may present with leukopenia, thrombocytopenia, elevated transaminases, and elevated lactate dehydrogenase.

“Rare cases of Anaplasmosis showcase post-infectious complications such as demyelinating polyneuropathy and brachial plexopathy,” wrote the authors. “However, this patient’s presentation of anaplasmosis with new onset trigeminal neuralgia appears to be unique and rare.”

The authors urge clinicians to consider Lyme disease, anaplasmosis, and Ehrlichia in “a patient presenting to the hospital with non-specific symptoms of fever, myalgia, and headache in tick endemic areas.”

References:
  1. LeDonne MJ, Ahmed SA, Keeney SM, Nadworny H. Trigeminal Neuralgia As the Principal Manifestation of Anaplasmosis: A Case Report. Cureus. Jan 2022;14(1):e21668. doi:10.7759/cureus.21668
  2. https://medlineplus.gov/trigeminalneuralgia.html

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For more:

Category:

Anaplasmosis, research, Testing, Treatment

US Government Agent Testing: Biological, Chemical Agents Making Thousands Sick – Tick-borne Illness

https://rumble.com/vq4k3f-us-government-agent-testing-biological-chemical-agents-making-thousands-sic.  Video Here (Approx. 15 Min)

Karma Newberry is the founder and CEO of Discovery Hydrovac, which serves the oil and gas industry. She’s become a successful businesswoman in a male-dominated field despite years of severe health problems that she’s suffered from for decades. At her peak, Karma was on more than 25 medications. After years of searching and consulting with more than 15 doctors, Karma says a physician finally checked her for tick-borne illnesses like Lyme disease. And the results shocked her: Karma had a bunch of them, including diseases that aren’t even present in the U.S. So how would she have them?

Those involved with classified human domain bioweapon testing:

  • Pfizer
  • Merck
  • Boeing
  • General Mills
  • University of Utah
  • DOD
  • Health Insurance Companies
  • Utah Medical facilities

Please remember that Fauci was at the helm for this entire history.

Find Newberry on Telegram (you have to download it)

Also here:  Lyme Justice

For more:

Category:

Activism, Lyme, Testing

La Crosse Virus – 2nd Most Common US Virus Spread by Mosquitoes

https://theconversation.com/la-crosse-virus-is-the-second-most-common-virus-in-the-us-spread-by-mosquitoes-and-can-cause-severe-neurological-damage-in-rare-cases

La Crosse Virus is the Second-Most Common Virus in the US Spread by Mosquitoes – and Can Cause Severe Neurological Damage in Rare Cases

By Rebecca Trout Fryxell, Assoc, Professor of Medical and Veterinary Entomology, University of Tennessee

Sept. 9, 2022

For the Laudick family of Greensburg, Indiana, life forever changed on Aug. 5, 2013. That was the day 4-year-old Leah Laudick told her mom, Shelly, that she had a bad headache.

Two days later, Leah was hospitalized nearby with worsening headaches and a slightly elevated white blood cell count. She slept for most of the day and by Aug. 9 was largely unresponsive.

That day, during her transfer to Peyton Manning Children’s Hospital in Indianapolis, Leah had her first of several seizures. Doctors were unable to identify her illness – tests for diseases like meningitis, Rocky Mountain spotted fever and herpes simplex all came back negative.

One day later, on Aug. 10, Leah’s brain activity stopped. That evening she passed away in the arms of her grieving parents.  (See link for article)

_________________

**Comment**

A Bill Gates funded factory breeds 30 million mosquitoes to release in 11 countries and genetically modified mosquitoes are now vaccinating humans.

What could possibly go wrong?

Mainstream media & medicine will not connect the fact that millions of mosquitoes are being released all over the world with subsequent changes in ecology, disease transmission, human/animal health, the fact DARPA is involved and where “toxicity is health, and the old crazy is the new normal.”  BTW: it’s happening in Lymeland too.

Just repeat “It’s safe and effective.”

SUMMARY:

  • The only reason we know about this case is due to Leah’s father emailing the author (an associate professor of entomology) asking how he could help with her work and agreeing to tell their story.
  • The family learned a few months after her death that La Crosse virus was the culprit.
  • While West Nile Virus makes up more than 90% of annual viral infections from mosquitoes or ticks, La Crosse is the next most prevalent virus causing 2% of mosqui or tickborne viral infections a year which extrapolates out to 50-150 cases per year.
  • Historically most cases occurred in the upper Midwest but the majority now occur in the southern Appalachia region.
  • Nobody knows why but there’s plenty of trollop about the climate
  • It is carried and transmitted primarily by the eastern tree-hole mosquito, Aedes triseriatus, a native species found throughout most of the Eastern U.S. This mosquito’s preferred habitat is places with obvious tree holes for female mosquitoes to deposit their eggs, such as hardwood forests.
  • It may also be transmitted by two exotic and invasive mosquito species: the tiger mosquito, Aedes albopictus, and the bush mosquito, Aedes japonicus.
  • It’s hard to diagnose because it looks similarly to the flu.
  • The only way to test for it is to send it to the completely and utterly corrupt CDC, which monopolizes testing and maligns any other labs or tests.
  • Cases tend to cluster in local communities so those successfully diagnosed can tell local doctors and officials it is present in their area.
  • Symptoms start with fever, fatigue, vomiting, and headache that lasts nearly 2 weeks.  Most recover; however, like West Nile, it is neuroinvasive and the immunocompromised can have severe cases which are typically discovered in the hospital after experiencing a seizure, coma, partial paralysis of one side, or an altered mental state.  Some experience long term neurological damage and in rare cases, death.
  • Similarly to Lyme/MSIDS, the best antidote is to prevent the bite in the first place:
    • get rid of outdoor objects that catch and contain water to reduce mosquito breeding
    • avoid mosquitoes by staying inside during peak hours in the early evening
    • use repellents like mosquito coils, and bug spray
    • wear light clothing

Category:

Activism, mosquitoes, Testing, Viruses

Overcome Bacterial & Viral Infections With SOT and RGCC Test

https://forumhealthfonddulac.com/masterclass/

Masterclass: Overcome Bacterial and Viral Infections with SOT Therapy and the RGCC Test

October 11th at 7pm ET

Are you or a loved one suffering from Lyme disease, shingles, hepatitis, Epstein-Barr virus or another viral or bacterial infection?

In this masterclass, Dr. Clayton Bell and Dr. Terri Beim will discuss how RGCC testing and SOT (Supportive Oligonucleotide Technique) is a highly effective and individualized method that can aid the body in overcoming bacterial and viral infections so you can feel like yourself again.

REGISTER FREE TODAY!
 

In This Masterclass You Will Learn

  • What is SOT therapy and RGCC testing?
  • How does SOT and RGCC testing work?
  • What are the benefits of SOT and how effective is it?
  • What are the side effects?
  • How does SOT address co-infections if they exist?
  • How long does it take to start feeling better?
The webinar is especially relevant for anyone suffering from:
  • Lyme disease
  • Shingles
  • Hepatitis B and C
  • Epstein-Barr virus
  • Cytomegalovirus
  • Coxsackie virus
  • Co-infections such as Babesia and Bartonella
  • HHV 1, 2 and 6
  • HPV 6, 11, 16 AND 18
  • HTLV1

About Terri M. Beim, ND

As a naturopathic practitioner for more than 20 years, Dr. Beim is first and foremost an educator. She practices at Forum Health Austin and spends an extensive amount of time with patients — understanding their medical history, body, chemistry, diet, lifestyle, load toxicity, and resulting health challenges — in effort to determine WHY their problems started in the first place. After seeking to understand, Dr. Beim educates her patients and offers personalized protocols that ensure positive health outcomes.
“Listening to and learning from patients is the only way to create an effective treatment plan. It must be personal; it must be holistic”.

Not only is Terri known for her extensive knowledge base in the field, but she brings overwhelming partnership, encouragement, and commitment to each patient’s journey of life-long health, wellness, and vitality.

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About Clayton Bell, MD, FAAFP, ABOIM

Dr. Clayton Bell’s medical practice combines cutting edge Functional Medicine with Integrative Medicine wellness techniques and ancient healing traditions from Ayurvedic Medicine. This synergistic and potent medicinal blend allows the patient to be heard and seen on multiple levels: physical, psychological, emotional, and spiritual. Dr. Bell has been shaped by his transformational personal and medical experiences both nationally and internationally. He has delivered babies in Maine, treated cholera epidemics in Haiti, taken a vision quest, ridden his bicycle across the country twice, and hiked across the Himalayas, Alps, Patagonia, New Zealand, and Kilimanjaro. These experiences solidified Dr. Bell’s medical philosophy that the common thread to optimizing vitality and health lies with activating, supporting, and empowering the person to heal themselves from the inside out.  Together, you and Dr. Bell will co-create a personalized wellness plan which will address your concerns and maximize your health.   

For more on SOT:

For more on the RGCC test:

Category:

Activism, Lyme, Testing, Treatment