Archive for the ‘research’ Category

Lone Star Ticks in Wisconsin

https://academic.oup.com/jme/article-abstract/doi/10.1093/jme/tjw218/2952769/Occurrence-of-Amblyomma-americanum-Acari-Ixodidae?redirectedFrom=fulltext

Occurrence of Amblyomma americanum (Acari: Ixodidae) and Human Infection With Ehrlichia chaffeensis in Wisconsin, 2008–2015
Megan Christenson, Xia Lee, Scott Larson, Diep Hoang Johnson, Julia Jensen, Megan Meller, Susan Paskewitz

Abstract
Because of the increasing incidence of human ehrlichiosis in Wisconsin, we assessed reports of human infections by Ehrlichia chaffeensis and the distribution of its vector, the lone star tick (Amblyomma americanum (L.)). From 2008 through 2015, 158 probable and confirmed human cases of E. chaffeensis infections were reported to the Wisconsin Department of Health Services. Five cases without travel history outside of Wisconsin were confirmed as E. chaffeensis by polymerase chain reaction. Surveillance for the vector occurred from 2008 through 2015 and was based on active and passive methods, including examination of white-tailed deer, collections from live-trapped small mammals, submissions of ticks removed from wild and domestic animals through the Wisconsin Surveillance of Animals for Ticks (SWAT) program, digital or physical submissions by the public to the University of Wisconsin Insect Diagnostic or Medical Entomology laboratories, and active tick dragging. More than 50 lone star ticks (46 adults, 6 nymphs, and 1 larva) were identified. Lone star ticks were more commonly found in south central Wisconsin, particularly in Dane County, where discovery of more than one life stage in a single year indicates possible establishment.

Category:

research, Ticks

Spread of TBI’s in Michigan

https://academic.oup.com/ofid/article-lookup/doi/10.1093/ofid/ofw269

Geographic Expansion of Lyme disease in Michigan, 2000-2014

Paul M. Lantos, Jean Tsao, Lise E. Nigrovic, Paul G. Auwaerter, Vance Fowler, Felicia Ruffin, Erik Foster, and Graham Hickling
Open Forum Infectious Diseases (2017) ofw269. Published 09 January 2017.

Abstract

Background.

Most Lyme disease cases in the Midwestern United States are reported in Minnesota and Wisconsin. In recent years, however, a widening geographic extent of Lyme disease has been noted with evidence of expansion eastwards into Michigan and neighboring states with historically low incidence rates.

Methods.

We collected confirmed and probable cases of Lyme disease from 2000 through 2014 from the Michigan Department of Health and Human Services, entering them in a geographic information system. We performed spatial focal cluster analyses to characterize Lyme disease expansion. We compared the distribution of human cases with recent Ixodes scapularis tick distribution studies.

Results.

Lyme disease cases in both the Upper and Lower Peninsulas of Michigan expanded more than five-fold over the study period. While increases were seen throughout the Upper Peninsula, the Lower Peninsula particularly expanded along the Indiana border north along the eastern shore of Lake Michigan. Human cases corresponded to a simultaneous expansion in established I. scapularis tick populations.

Conclusions.

The geographic distribution of Lyme disease cases significantly expanded in Michigan between 2000 and 2014, particularly northward along the Lake Michigan shore. If such dynamic trends continue, Michigan can expect a continued increase in Lyme disease cases, as may neighboring areas of Indiana, Ohio, and Ontario, Canada.

Category:

Lyme, research, Ticks

Bm & TBI’s in NY State

http://www.sciencedirect.com/science/article/pii/S1877959X17300158

Detection of Borrelia miyamotoi and other tick-borne pathogens in human clinical specimens and Ixodes scapularis ticks in New York State, 2012-2015.

Wroblewski D, Gebhardt L, Prusinski MA, Meehan LJ, Halse TA, Musser KA.

Ticks and Tick-borne Diseases, online first, 2017 Jan 16. pii: S1877-959X(17)30015-8.

Abstract

Borrelia miyamotoi (Bm) is a recently emerging bacterial agent transmitted by several species of ixodid ticks. Diagnosis of Bm infection can be challenging, as the organism is not easily cultivable.

We have developed and validated a multiplex real-time PCR to simultaneously identify Bm infection and the agents causing human granulocytic anaplasmosis and human monocytic ehrlichiosis, Anaplasma phagocytophilum and Ehrlichia chaffeensis, respectively. The assay is 100% specific; highly sensitive, detecting 11 gene copies of Bm DNA in both whole blood and cerebral spinal fluid; and provides rapid results in less than two hours.

A retrospective study of 796 clinical specimens collected between the years 2012 and 2014 and a prospective study of 366 clinical specimens were performed utilizing this novel assay to evaluate the frequency of Bm infection in New York State (NYS). Eight clinical specimens (1%) were found to be positive for Bm, 216 were positive for A. phagocytophilum, and 10 were positive for E. chaffeensis.

Additionally, we tested 411 I. scapularis ticks collected in NYS during 2013 and 2014 in a separate multiplex real-time PCR to determine the prevalence of Bm, A. phagocytophilum, Borrelia burgdorferi s.s., and Borrelia species. Our results indicated rates of 1.5%, 27%, 19.7%, and 8.8% respectively.

The ability to monitor both the frequency and geographic distribution of Bm cases and the prevalence and geographic distribution of Bm in ticks will help create a better understanding of this emerging tick-borne pathogen.

Category:

Anaplasmosis, Lyme, research, Ticks

Study Showing Contaminated Vaccines

http://www.thevaccinereaction.org/2017/02/dirty-vaccines-new-study-reveals-prevalence-of-contaminants/

A new article written by Celeste McGovern reveals that every human vaccine tested (over 30) was contaminated by unsafe levels of metals such as lead, tungsten, gold, and chromium, and debris, including red blood cells, and is linked to cancer and autoimmune disease.

The study published in the International Journal of Vaccines and Vaccination shows micro- and nano-sized particulate matter of inorganic elements not declared in the products’ ingredients lists.

One researcher observed that the unusual chemical compositions look like burnt waste.

But McGovern states:

“There are dozens of other ‘incidents’ which would better be called acts of criminal negligence, including:

*The polio vaccine doled out between 1955 and 1963 was contaminated with simian virus 40 (SV40) from monkey kidney cells used to produce the vaccine. It’s been linked to the growing epidemic of cancer.

*In 2007, Merck & Company, Inc. recalled 1.2 million doses of Hib vaccines due to contamination with bacteria called cereus, a potentially lethal food-poisoning bug.

*In 2009, more than 40,000 doses of a meningitis C vaccine for babies were withdrawn from the British market when they were found to be contaminated with blood-poisoning bacteria, S aureus.

*In 2010, deep sequence analysis of eight different live attenuated virus vaccines revealed unexpected viral sequences in three of them: retrovirus avian leukosis was found in a measles vaccine, a virus similar to simian retrovirus was identified in Rotateq anti-diarrhea vaccine developed by CDC consultant Paul Offit, and the entire genome sequence of porcine cirovirus1 was found in Rotarix leading the FDA to suspend the rotavirus vaccine.

*In 2014, The U.S. Food and Drug Administration ordered GlaxoSmithKline to review the manufacturing operation of its flu vaccine when it found microbiological contamination of products purporting to be sterile.

*In 2013, Merck & Company, Inc. recalled one batch of Gardasil when glass particles were discovered in several phials.

*Recently it was reported that Sanofi Pasteur refused to recall its ActHIB vaccine for babies, even though it knew it was contaminated with glass shards. The FDA didn’t object.”

McGovern claims even when vaccine manufacturers are given warnings, nothing changes since they are immune from lawsuits.

 

Category:

Activism, research, vaccines

Methods of Tick Removal: A Systematic Review of the Literature

http://amj.net.au/index.php?journal=AMJ&page=article&op=view&path%5B%5D=2804

METHODS OF TICK REMOVAL: A SYSTEMATIC REVIEW OF THE LITERATURE

Nikki Coleman, Stephen Coleman

ABSTRACT

Background
An increase in tick borne diseases in Australia has seen an interest in appropriate removal of ticks (order Ixodida) in order to prevent anaphylaxis, allergy and transmission of tick borne diseases.

Aims
A systematic review of peer-reviewed literature to determine what method of tick removal should be promoted in terms of preventing future health complications.

Methods
Thematic synthesis was used in two stages: – tick removal studies conducted on animals and humans were examined and the conclusions from all of these studies were compared, in order to ascertain the best tick removal method in relation to prevention of future medical problems (including tick bite allergy and transmission of infection).

Conclusion
This systematic review documents the best method of tick removal based on scientific and medical studies between 1985 and 2016. It concludes that the best method is to remove the tick as soon as possible after it is detected, using either fine-tipped tweezers or a reputable commercially produced tick removal tool to pull the tick away from the site of attachment. Some methods of removal, such as applying chemicals like petroleum jelly, alcohol, or nail polish to the tick, have been discredited. Other methods of removal, such as freezing, while promising, have not yet been scientifically validated.
Full Text: PDF

Category:

research, Ticks