Are There Hidden Genes in DNA/RNA Vaccines?
- 1Department of Biochemistry, Sanger Building, University of Cambridge, Cambridge, United Kingdom
- 2Department of Biology and Ecology, University of Ostrava, Ostrava, Czechia
- 3Department of Physics, University of Ostrava, Ostrava, Czechia
Due to the fast global spreading of the Severe Acute Respiratory Syndrome Coronavirus – 2 (SARS-CoV-2), prevention and treatment options are direly needed in order to control infection-related morbidity, mortality, and economic losses. Although drug and inactivated and attenuated virus vaccine development can require significant amounts of time and resources, DNA and RNA vaccines offer a quick, simple, and cheap treatment alternative, even when produced on a large scale. The spike protein, which has been shown as the most antigenic SARS-CoV-2 protein, has been widely selected as the target of choice for DNA/RNA vaccines. Vaccination campaigns have reported high vaccination rates and protection, but numerous unintended effects, ranging from muscle pain to death, have led to concerns about the safety of RNA/DNA vaccines. In parallel to these studies, several open reading frames (ORFs) have been found to be overlapping SARS-CoV-2 accessory genes, two of which, ORF2b and ORF-Sh, overlap the spike protein sequence. Thus, the presence of these, and potentially other ORFs on SARS-CoV-2 DNA/RNA vaccines, could lead to the translation of undesired proteins during vaccination. Herein, we discuss the translation of overlapping genes in connection with DNA/RNA vaccines. Two mRNA vaccine spike protein sequences, which have been made publicly-available, were compared to the wild-type sequence in order to uncover possible differences in putative overlapping ORFs. Notably, the Moderna mRNA-1273 vaccine sequence is predicted to contain no frameshifted ORFs on the positive sense strand, which highlights the utility of codon optimization in DNA/RNA vaccine design to remove undesired overlapping ORFs. Since little information is available on ORF2b or ORF-Sh, we use structural bioinformatics techniques to investigate the structure-function relationship of these proteins. The presence of putative ORFs on DNA/RNA vaccine candidates implies that overlapping genes may contribute to the translation of smaller peptides, potentially leading to unintended clinical outcomes, and that the protein-coding potential of DNA/RNA vaccines should be rigorously examined prior to administration.
**UPDATE March 25, 2022**
Here’s some important comments on the paper from James Lyons Weiler, PhD:
“They presume there is not issue with the ORFs on the negative sense strand, apparently unaware of the studies that show the mRNA is, in fact, incorporated into the tissue of fast-dividing cells.
Proteins not found in the human genome expressed by human cells will lead to cytotoxic t-cells attacking them and initiating cell death. Therefore, cellular damage and organ damage will occur due to these unintended, preventable proteins translated by the protein-producing machinery of our cells.
Read the paper’s conclusions. Clearly, these steps should have been done before unleashing this biologic on the human population
It is utterly unacceptable that Moderna and Pfizer did not catch this, and it is similarly unacceptable that FDA and CDC organizations such a VRBPAC and ACIP did not catch this.
There is no plan to update the Moderna and Pfizer vaccines to disrupt these open reading frames – or to remove the unsafe epitopes that can lead to autoimmunity against proteins, including many of those in our immune systems.
It’s time we take the bad news that the public knows and understands – how the rush to vaccines was not even close to “science”, but instead was mayhem.”
For VAERS data and a mounting list of adverse reactions & deaths reported after COVID shots: https://madisonarealymesupportgroup.com/2020/12/21/warning-3150-injuries-in-1st-week-of-covid-vaccines-among-american-healthcare-workers-pregnant-women-included/