Archive for the ‘research’ Category

Imaging Shows Early Vascular Interactions of Borrelia in Skin

https://www.nature.com/articles/s41467-025-64326-w

Open access

Published: 

Targeted volume imaging reveals early vascular interactions of Lyme disease pathogen in skin

Nature Communications volume 16, Article number: 9330 (2025) Cite this article

Abstract

Although the contours of the dissemination pathways of human pathogenic spirochetes in the vertebrate hosts are known, detailed high-resolution information on these processes remain lacking. In this study, we establish an efficient serial block-face scanning electron microscopy workflow incorporating semi-automatic AI-driven segmentation to investigate the architecture of early events following the deposition of Borrelia burgdorferi at the tick bite site in mice. We capture evidence of Borrelia penetrating the lymphatic endothelium via both transcellular and paracellular routes and observe its early presence within the lumen of the lymphatic vessel. The multistep process of transcellular migration is documented in detail, showing sequential invagination and encasement of shorter Borrelia segments by the lymphatic endothelial cells during intravasation. Our findings reveal that the first contact of B. burgdorferi and blood vessels is not random but involves close interactions with pericytes. We also capture the infiltration of immune cells in the skin and their interactions with invading bacteria. Altogether, these observations suggest that Borrelia strategically targets vascular regions with lower mechanical resistance to breach the endothelial barrier, thereby enhancing its dissemination.

Category:

Lyme, research

Case Report of Male With Anaplasmosis

https://www.cureus.com/articles/416304-relative-bradycardia-in-a-61-year-old-male-with-anaplasmosis-a-case-report#!/

Relative Bradycardia in a 61-Year-Old Male With Anaplasmosis: A Case Report

Jessica A. James • Melissa Brown • Samuel M. Segal • Maria Gutierrez-Castillo

Published: October 17, 2025

DOI: 10.7759/cureus.94785 

Open Access
Peer-Reviewed

Cite this article as: James J A, Brown M, Segal S M, et al. (October 17, 2025) Relative Bradycardia in a 61-Year-Old Male With Anaplasmosis: A Case Report. Cureus 17(10): e94785. doi:10.7759/cureus.94785

Abstract

Human granulocytic anaplasmosis (HGA), or anaplasmosis, is a tick-borne illness caused by Anaplasma phagocytophilum, a gram-negative intracellular bacterium. A. phagocytophilum is primarily transmitted by Ixodes scapularis in the northeast United States and by Ixodes pacificus in California. Presenting symptoms typically include fever, chills, malaise, headache, myalgia, and rarely a rash. This case describes a 61-year-old Black male with a complex medical history, including prior tick-borne and arboviral infections (Lyme disease, dengue fever, and chikungunya), hypertension, mixed hyperlipidemia, bilateral carotid artery dissection, gastroesophageal reflux disease, atrial fibrillation with rapid ventricular response, and current tobacco use. This patient presented to an emergency department in upstate New York with a fever, fatigue, constipation, myalgia, and night sweats. Throughout the patient’s hospital course, he maintained a state of relative bradycardia. The patient reported that he had returned from Haiti and the Dominican Republic two weeks prior to presentation in the emergency department and received several mosquito bites while abroad. Initial guideline-based empiric treatment was started with doxycycline due to suspicion of tick-borne illness, given his history of Lyme disease and his onset of symptoms while in upstate New York. Treatment was continued to complete a 14-day course after confirming the diagnosis of anaplasmosis by PCR testing of whole blood. After completing treatment with doxycycline, the patient’s symptoms resolved completely. This case illustrates a unique finding of relative bradycardia and fever of unknown origin in the context of recent international travel and history of tick-borne and arboviral infections.

 

Category:

Anaplasmosis, Heart Issues, Lyme, mosquitoes, research, Uncategorized

How the CDC Validates Chronic Lyme is All Smoke & Mirrors

https://www.change.org/p/the-us-senate-calling-for-a-congressional-investigation-of-the-cdc-idsa-and-aldf/u/34076105?

Progress Toward Understanding Infection-Associated Chronic Conditions and Illnesses

Carl TuttleHudson, NH, United States

Nov 19, 2025

This letter was written after seeing the following Facebook post from “Tick Boot Camp”

CDC Validates Chronic Lyme
https://www.facebook.com/photo/?fbid=1392710922860645&set=a.445348080930272

Carl Tuttle

———- Original Message ———-
From: CARL TUTTLE <runagain@comcast.net>
To: afiore@cdc.gov
Cc: dpd1@cdc.gov, Matthew_Kuehnert@mtf.org, ebelay@cdc.gov, richard.bradbury@jcu.edu.au
Date: 11/19/2025 9:06 AM EST
Subject: Progress Toward Understanding Infection-Associated Chronic Conditions and Illnesses

Emerging Infectious Diseases  Volume 31, Supplement—October 2025
 
Progress Toward Understanding Infection-Associated Chronic Conditions and Illnesses
Anthony E. Fiore
https://wwwnc.cdc.gov/eid/article/31/14/25-1187_article

Excerpt:

“Complicating the frustrated patient’s predicament, clinicians attempting unproven treatments (e.g., repeated antibiotic courses) might exacerbate illness by introducing additional risks or temporarily masking potentially treatable causes.”

To: Anthony Fiore, MD, MPH,

In 2016 Dr. Paul Auwaerter, past president of the Infectious Diseases Society of America coauthored a study revealing the persister form of Borrelia burgdorferi resistant to antibiotics.

Here is a timeline of events:

2015

Standard antibiotic treatment for Lyme disease does not kill persistent Borrelia bacteria.
http://droopyyoupi.blogspot.com/2015/08/standart-antibiotic-treatment-for-lyme.html

Excerpt:

-What has tuberculosis and Borrelia burgdorferi in common? In the late stage of the disease occurs persistent (tolerant) bacteria, which essentially means that the bacteria lasts and lasts and lasts. They protect themselves against antibiotics and are difficult to treat.

– Both Borrelia burgdorferi and tuberculosis is relatively easy to cure in the early stages, even with the use of one antibiotic. In the late stage it is impossible to cure the disease with the same type of treatment in the acute phase, said Dr. Ying Zhang when he visited the year NorVect conference.

-Dr. Ying Zhang is a professor at the Department of Molecular Microbiology and Immunology at the Johns Hopkins Bloomberg School of Public Health

-Two days after NorVect conference, published Dr. Ying Zhang’s latest research Identification of new compounds with high activity against stationary phase Borrelia burgdorferi from the NCI compound collection.

2016

A Drug Combination Screen Identifies Drugs Active against Amoxicillin-Induced Round Bodies of In Vitro Borrelia burgdorferi Persisters from an FDA Drug Library
Jie Feng 1, Wanliang Shi 1, Shuo Zhang 1, David Sullivan 1, Paul G Auwaerter 2, Ying Zhang 1
https://pubmed.ncbi.nlm.nih.gov/27242757/

Abstract

Under experimental stress conditions such as starvation or antibiotic exposure, Borrelia burgdorferi can develop round body forms, which are a type of persister bacteria that appear resistant in vitro to customary first-line antibiotics for Lyme disease. To identify more effective drugs with activity against the round body form of B. burgdorferi, we established a round body persister model induced by exposure to amoxicillin (50 μg/ml) and then screened the Food and Drug Administration drug library consisting of 1581 drug compounds and also 22 drug combinations using the SYBR Green I/propidium iodide viability assay. We identified 23 drug candidates that have higher activity against the round bodies of B. burgdorferi than either amoxicillin or doxycycline.

2022

Nitroxoline Drug Combinations Are More Active Than Lyme Antibiotic Combination and Can Eradicate Stationary-Phase Borrelia burgdorferi
Alvarez-Manzo, Hector S.1; Zhang, Yumin1; Zhang, Ying2,✉
https://journals.lww.com/imd/fulltext/2022/09000/nitroxoline_drug_combinations_are_more_active_than.7.aspx

Abstract

Lyme disease (LD), caused by Borrelia burgdorferi, is the most common vector-borne disease in the United States and Europe. Despite the standard 2–4 weeks’ antibiotic treatment, approximately 10%–20% of patients will develop posttreatment LD syndrome, a condition that is poorly understood. One of the probable causes is thought to be the presence of B. burgdorferi persister forms that are not effectively killed by the current LD antibiotics. In this study, we evaluated nitroxoline, an antibiotic used to treat urinary tract infections, for its activity against a stationary-phase culture enriched with persister forms of B. burgdorferi. Nitroxoline was found to be more active than doxycycline and equally active as cefuroxime (standard LD antibiotics) against B. burgdorferi. Importantly, the nitroxoline two-drug combinations nitroxoline + cefuroxime and nitroxoline + clarithromycin, as well as the nitroxoline three-drug combination nitroxoline + cefuroxime + clarithromycin, were as effective as the persister drug daptomycin-based positive control three-drug combination cefuroxime + doxycycline + daptomycin, completely eradicating stationary-phase B. burgdorferi in the drug-exposure experiments and preventing regrowth in the subculture study. Future studies should evaluate these promising drug combinations in a persistent LD mouse model.

Dr. Fiore… This is the missing research that should have been conducted early in the discovery phase of the disease but as we now know, all the eggs were put into the vaccine basket while a campaign was orchestrated to discredit the sick and disabled patient population along with the courageous clinicians attempting to help these patients. https://pubmed.ncbi.nlm.nih.gov/21867956/

What has been deceitfully established here in the US is wreaking havoc globally. Example:
Lyme disease: Australians ‘being treated worse than a dog riddled with mange’, Senator John Madigan says
https://www.abc.net.au/news/2016-01-11/lyme-disease-treatment-in-australia-criticised-by-john-madigan/7080708

This research is being suppressed as the disabled Lyme patient population around the globe remain sick indefinitely. (Three decades and counting)

Carl Tuttle
Independent Researcher
Hudson, NH

Cc: D. Peter Drotman, MD, MPH Editor-in-Chief

Category:

Activism, Lyme, research, vaccines

Anomalous Amyloid Microclots Found in 100% of COVID Vaxxed

https://www.thefocalpoints.com/p/breaking-study-anomalous-amyloid?

BREAKING STUDY: Anomalous Amyloid Microclots Found in 100% of the COVID-19 Vaccinated

In a cohort that was 94% vaccinated, every participant had amyloid microclots —the same pathology behind the large white fibrous clots now being pulled from corpses worldwide.

Nov 17, 2025

by Nicolas Hulscher, MPH

A new peer-reviewed study has quietly revealed one of the most consequential biological findings of the pandemic era — and the authors never acknowledge it: Every single vaccinated participant in the study had fibrinolysis-resistant, ThT-positive amyloid microclots circulating in their blood.

Hidden in the supplementary tables is a demographic and biochemical pattern that completely reframes the paper:

  • 94% of all participants were vaccinated
  • 100% of these vaccinated individuals had amyloid microclots — including every “healthy control”

The condition labeled “Long COVID” occurred almost entirely in a heavily vaccinated population, without any laboratory confirmation of prior SARS-CoV-2 infection. In reality, the study is observing Long VACCINE pathology, not Long COVID.

And because the authors’ own mechanistic experiments show that purified spike protein alone produces these amyloid, fibrinolysis-resistant clots, the implications are profound. (See link for article and graphs)

_______________

**Comment**

Not quite sure how much more damning evidence is needed, but these things needed to be thrown in the dust bin years ago....

For more:

Category:

Activism, research, vaccines

First Reported Death Supposedly By AGS

Before you read the following article, it bears repeating that researchers and doctors are making a CAUSAL connection between lone star tick bites and Alpha Gal Syndrome (AGS) meat allergy despite the fact there are people with AGS who were not bitten by the tick.

Further, journalist Jon Rappoport takes this even further by stating the very same type of causal link used for AGS is not accepted at all for ‘vaccines’ causing subsequent injuries.  

Both conditions are self reported and in the following story, the patient remembers being bitten by chiggers not a tick…..

https://www.nbcnews.com/health/health-news/first-death-reported-meat-allergy-caused-tick-bite

First death reported from meat allergy caused by tick bite

After months of investigation, researchers confirmed that a New Jersey man died of a tickborne allergy called alpha-gal syndrome after eating a hamburger.
 
By Aria Bendix

A 47-year-old airline pilot from New Jersey is the first person known to have died from alpha-gal syndrome, a red meat allergy caused by a tick bite.

Researchers at the University of Virginia School of Medicine publicly reported the cause of death Wednesday after months of investigation. Their findings were published in the Journal of Allergy and Clinical Immunology: In Practice.

The man’s death had previously been seen as a mystery, since medical examinations showed no evidence of a heart attack or other life-threatening issues.

According to the researchers, the man started feeling sick four hours after consuming a hamburger at a barbecue in September 2024. When he returned home, he was well enough to mow the lawn and read the paper. But shortly after 7:30 p.m. that day, the man’s son found him unconscious on the bathroom floor with vomit around him. An autopsy ruled that his death was sudden and unexplained.

Two weeks earlier, the man had become ill after eating a steak dinner on a camping trip with his wife and children. The researchers said he woke up at 2 a.m. with severe diarrhea, vomiting and stomach pain, and later told one of his sons that he thought he was going to die. However, he and his wife were uncertain of what had happened, so they decided not to consult a doctor.

“The tragedy is that they didn’t think of that episode as anaphylaxis, and therefore didn’t connect it to the beef at the time,” said Dr. Thomas Platts-Mills, an allergist at the University of Virginia School of Medicine who discovered alpha-gal syndrome and diagnosed the New Jersey man’s case.

(See link for article and newsvideo)

________________

**Comment**

The cause of the allergic reaction did not show up on his autopsy.

But when a family friend who was a doctor talked to the man’s wife, they wondered if it could be AGS.  Another doctor who knew the family friend worked with the wife for blood testing.  The highest level for anaphylaxis this doctor had ever seen on a survivor is 100.  The deceased’s level was 2,000.

A few interesting points:

  • According to the good doctor, food allergy deaths are really rare and happen in people with underlying asthma or ‘some other kind of medical condition.’  The good doctor didn’t mention ‘vaccines’, but since the deceased was a pilot and they were mandated to get the experimental clot shots, I’m betting he got it – and that indeed could have been his precipitating ‘medical condition,’ or the big fat elephant in the room everyone blithely ignores.
  • The good doctor said a recent bite could boost AGS.  The wife said the deceased had been bitten by chiggers, however, researchers now suspect the bites came from lone star tick larvae. 
  • The article makes sure to only push the fear narrative that it’s solely ticks, and roving deer due to climate change, completely ignoring the fact people get AGS without any tick bite whatsoever, implicating ‘vaccines,’ and the fact our own government has been working on ticks for decades and dropping them from airplanes.  

Category:

Activism, Alpha Gal Meat Allergy, research, Ticks