Proof of Borrelia Persistence

The following work by microbiologist Thomas Grier and pathologist Alan MacDonald should prove to the CDC that Borrelia can undoubtably persist inside the human body.

“For years pathologists stained for amyloid to make the diagnosis, but when the same plaques in a serial sectioning were stained for B burgdorferi or B miyamotoi, individual classical spirochetes could be seen, but more commonly a biofilm of Borrelia could be seen in the same plaques. Borrelia is a brain-pathogen.”


Above:  “Borrelia miyamotoi using Alan MacDonald’s FISH stain. This is the first documented case of Bm in Minnesota.”


Above and below:  “Since Fluorescence FISH stain target species specific nucleotide sequences, it is species specific. Spirochetes are excellent subjects because the Borrelia DNA is associated with the cell wall and membrane. Another probe can isolate cytoplasmic DNA thus giving a 3-D look to it. What a beautiful killer.”


Below:  “This patient was told for 22 years that she had MS, our work on MS allowed us to determine that this brain MRI was probably a result of Lyme. Do you see the markers? This Philadelphia woman made a very good recovery from her disability and went back to work.”

Below:  “This is Borrelia burgdorferi in the brain of a man sick for two decades. The others in red were Borrelia miyamotoi. Another finding was even after treatment he had neutrophils in the brain, and indication of a breakdown of the BBB and inflammation. He was sicker than doctors realized!”


“The last photo taken of Grier’s friend Scott from Twig MN.  His deathbed video is all about helping other Lyme victims.”  Scotty was a 57 year old Lumberman who lived his whole life in the forest.  Scotty was seronegative for Lyme but was bedridden with pain and neurolyme and was treated for 7+ years with aggressive antibiotics.  His brain autopsy revealed several surprises and live active Borrelia burgdorferi.”  


Contact Thomas Grier M.S. at to ask him about helping fund his research.

For more:  Conclusions: Using multiple corroborative detection methods, we showed that patients with persistent Lyme disease symptoms may have ongoing spirochetal infection despite antibiotic treatment, similar to findings in non-human primates. The optimal treatment for persistent Borrelia infection remains to be determined.  For the first time, Garg et al. show a 85% probability for multiple infectionsincluding not only tick-borne pathogens but also opportunistic microbes such as EBV and other viruses….In addition to tick-borne co-infections and non-tick-borne opportunistic infections, pleomorphic Borrelia persistent forms may induce distinct immune responses in patients by having different antigenic properties compared to typical spirochetes32,33,34,35. Nonetheless, current LD diagnostic tools do not include Borrelia persistent forms, tick-borne co-infections, and non-tick-borne opportunistic infections.  Microscopy, Culture or PCR-verified cases of persistent [seronegative] Lyme Borreliosis