Archive for the ‘research’ Category

Alumnus Works to Protect People From West Nile Virus, Lyme Disease

http://www.etownian.com/features/alumnus-works-to-protect-people-from-west-nile-virus-lyme-disease/

Alumnus works to protect people from West Nile Virus, Lyme disease

APRILLE MOHN FEBRUARY 23, 2019

Photo from Wikimedia Commons

Feb. 18, Elizabethtown College alumnus Jon Bachman, ‘17, returned to Elizabethtown College to speak to current students about his career as an aquatic biologist with the Pennsylvania Department of Environmental Protection (PA DEP).

Bachman is part of a team researching and working to increase awareness of arboviruses. Arboviruses are viruses that are transmitted by mosquitoes, ticks or other arthropods. Bachman and his colleagues work to protect people from tick and mosquito-borne pathogens, most prominently West Nile Virus (WNV), and more recently, Lyme disease.

The DEP is studying WNV and the mosquitoes that carry it, which according to Vector Disease Control International, are primarily Culex pipiens, Culex tarsalis, and Culex quinquefasciatus.

Bachman and his team look at mosquito habitat so larvae can be suppressed and killed. They also set traps so when a female mosquito lays eggs in their trap they are able to collect the samples. They then smash the eggs and conduct polymerase chain reaction (PCR) on the DNA in them. PCR is a process that can make many copies of specific DNA strands. By replicating the DNA they are able to see if the virus is carried by the eggs they collected.

Based on where large traces of the West Nile Virus is found the team determines where they’re going to spray for adult mosquitoes in order to prevent the spread of the virus.

This past year has had the most WNV positive mosquitoes. There were 7,500 positive samples, which was much higher than previous years. The second highest positives found in a year was in 2012 when around 6,000 positive samples were found.

Some important information about the disease Bachman shared was that WNV is not spread between people—birds are the reservoir species for the virus, meaning that the mosquitoes must first get it from birds before they can transmit it to any humans.

Another distinction is that the mosquitoes which often carry West Nile Virus are not the large groups of mosquitoes people encounter. Those are typically the inland floodwater species of mosquito, or Aedes vexans.

Floodwater mosquito eggs often hatch all at once because the adult female mosquito lays eggs that dry out and don’t hatch until they get wet. On years with large amounts of flooding, such as this year, all the eggs get wet and all hatch at same time.

There are 62 species of Mosquitoes in Pennsylvania, but the vast majority do not carry West Nile Virus.

“If you’re being swarmed with mosquitoes they are not the ones carrying the disease,” Bachman said. “If you’re getting swarmed you don’t have to worry about getting the virus. It’s the one mosquito you don’t feel—that’s how you get the virus.”

This year, Bachman’s team has begun to do research on Ixodes scapularis, more commonly known as the deer tick, because of the Lyme disease they carry. Pennsylvania is the worst state for Lyme disease almost, if not every, year. For this reason, the DEP’s research is being funded by the Center for Disease Control (CDC) through the Department of Health.

Bachman’s team is trying to find 50 adult deer ticks in each county in Pennsylvania to determine the rate of Lyme disease for each region of the state.

According to Bachman, it takes a lot of work to catch 50 ticks.They have to look at three different sites and so they try to pick sites they know people will be at.

Bachman said he has the most luck along the edges of soccer fields with woodlines.

Each search is different, as well. Bachman spent six days in Fulton County, PA and was unable to catch a single tick, and yet in the next county over, Bedford County, he was able to catch over 100 ticks in half an hour.
Bachman said he has a “very, very unique job. It’s more of a public health job.”

To determine the best ways to control West Nile Virus and Lyme disease, he and his peers need to understand the habitats and life cycles of the organisms that spread the illnesses.

Bachman was an environmental science major with a minor in political science. While his career is much more focussed on ideas from environmental science Bachman found his minor helpful to learn and understand environmental law and regulations.

Moreover, his political science minor taught Bachman more about public speaking, which is important as part of his career is to work with representatives of different countries who come to him for advice regarding mosquitoes and ticks.

While at Etown, Bachman was involved with the SEEDS Ecology Club. He was the club’s treasurer his senior year, which was the first year that the College had a chapter of the nationwide program.

Bachman also has a U.S. Army background as he was a member of the Army from 2005 to 2012. He first worked as an Infantry man and later he worked with hazardous materials, or hazmat.

This work in the Army with hazmat led Bachman to the job he held prior to his position with the DEP, which was a job as the hazmat supervisor at a Harley Davidson in York, PA.

One piece of advice Bachman had for students in regards to finding jobs after college is to study the jobs they are applying for closely. “Look into every detail of a job before you go to an interview,” Bachman said.

Bachman recommends students learn about as many fields as they can, and make themselves well rounded in preparation for finding a career.

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**Comment**

Please notice the alarming statement of where Bachman finds the most ticks:

Bachman said he has the most luck along the edges of soccer fields with woodlines.

Please educate your family, friends, neighbors, and school systems.  Wisconsin children should all be wearing permethrin treated shoes and socks at the very least as they are at risk.  For more:  https://madisonarealymesupportgroup.com/2018/05/27/study-conforms-permethrin-causes-ticks-to-drop-off-clothing/

Also, I’d like to see Wisconsin go back to ditch burning:  https://madisonarealymesupportgroup.com/2018/04/03/fire-good-news-for-tick-reduction/

For a similar topic in Wisconsin, see:  https://madisonarealymesupportgroup.com/2018/03/13/wed-nite-the-lab-talk-on-mosquitoes-ticks-disease/

Wisconsin has cases of West Nile, La Crosse Virus, & Jamestown Canyon Virus.
Wisconsin is 4th in the nation for Lyme disease.

The CDC says the cases are hugely underestimated – more like 30,000 cases per year in WI.  WI is a hotspot for newly emerging TBI – Anaplasma, Ehrlichia muris, borrelia miyamotoi (relapsing fever), Babesia divergens (in Michigan but Dr. Paskowitz feels it’s probably here too). Anaplasma seeing 400-600 cases a year in WI.  Again, much underreporting.

 

 

Seriously? More Research Utilizing Blood Testing For Bb When it Can’t Tell Active, Past, or Reinfection

A recent paper by Schutzer et al. concluded the following regarding serologic testing for Lyme disease:

“… serologic tests cannot distinguish active infection, past infection, or reinfection. Reliable direct-detection methods for active B. burgdorferi infection have been lacking in the past but are needed and appear achievable.”  https://madisonarealymesupportgroup.com/2018/10/12/direct-diagnostic-tests-for-lyme-the-closest-thing-to-an-apology-you-are-ever-going-to-get/

Yet, the following recent study attempts to use numbers based on these faulty blood tests to make testing results a wash between false positives and the number of unreported true positive cases.

https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(19)30086-2/pdf

Abstract

Objectives

To evaluate the impact of false positive IgM immunoblots on Lyme disease treatment and case reporting in a large healthcare system.

Methods

We obtained results of all Lyme disease serologic tests ordered at U.S. Air Force healthcare facilities in the United States between January 2013 and December 2017. We conducted chart reviews to adjudicate positive IgM immunoblots (from two-tier and independent testing) as true positives or false positives using established criteria, and we assessed whether these cases were reported to the U.S. Department of Defense surveillance system.

Results

Of the 18,410 serum tests (17,058 immunoassays and 1,352 immunoblots) performed on 15,928 unique persons, 249/1,352 (18.4%) IgM immunoblots were positive. After excluding repeat tests, insufficiently documented cases, and subjects with a history of Lyme disease, 212 positive IgM immunoblot cases were assessed. A total of 113/212 (53.3%) were determined to be false positives. Antibiotics were prescribed for Lyme disease for 97/99 (98.0%) subjects with a true positive test and 91/113 (80.5%) subjects with a false positive test. The number of false positive cases reported to the surveillance system was identical to the number of unreported true positive cases (n=44).

Conclusions

Lyme disease serological tests were overutilized in a large healthcare system, and positive results were frequently misinterpreted, leading to misdiagnosis and widespread antibiotic misuse. Underreporting of true positive cases was offset by overreporting of false positive cases, suggesting that the discrepancy between the reported incidence and true incidence of Lyme disease may not be as significant as previously assumed.

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**Comment**

Oh, there are discrepancies, alright.

Great example of the need for better testing.  How about direct testing which has been suppressed for decades?  https://madisonarealymesupportgroup.com/2017/12/13/suppression-of-microscopy-for-lyme-diagnostics-professor-laane/

https://madisonarealymesupportgroup.com/2019/02/27/should-lyme-patients-rico-lawsuit-include-yale/

https://madisonarealymesupportgroup.com/2018/05/15/news-release-on-57-1-million-lyme-disease-lawsuit-filed-against-cdc/

The study is a fantastic example of “garbage in, garbage out.”  If there isn’t a reliable test, this whole paper is worthless.

Regarding false results,

“If false results are to be feared, it is the false negative result which holds the greatest peril for the patient.” -Dr. Alan MacDonald

Quit trying to downplay this pandemic that is sweeping through like a modern-day plague.  It’s real, and it’s not going away.

The emperor has no clothes on!

More on testing:  https://madisonarealymesupportgroup.com/2018/09/12/lyme-testing-problems-solutions/  Excerpt:

One has to wonder why Yale didn’t want to use a test that they patented that would capture the vast majority of lyme patients.
HOWEVER AS I SEE IT IF THEY UTILIZED THIS TEST TO VALIDATE THEIR LYME VACCINE THE RESULTS WOULD SHOW THAT THEIR SO CALLED VACCINE WAS IN FACT THE OPPOSITE OF A VACCINE AND INDUCED THE VERY DISEASE IT WAS SUPPOSED TO PROTECT THE PERSON FROM, AS WAS THE CASE FOR MANY PEOPLE WHO WERE INJURED BY THE FIRST LYME VACCINE LYMERIX WHICH WAS TAKEN OFF THE SHELF.

I can not fathom any other reason why they would not use a test they owned that has 96% accuracy overall and 100% specificity.

The answer can only be fraud.

https://madisonarealymesupportgroup.com/2018/01/16/2-tier-lyme-testing-missed-85-7-of-patients-milford-hospital/

https://madisonarealymesupportgroup.com/2018/10/24/another-useless-study-showing-lyme-testing-is-abysmal/

https://madisonarealymesupportgroup.com/2018/09/08/whats-the-best-test-for-lyme-dr-rawls/

https://madisonarealymesupportgroup.com/2018/05/11/lyme-sci-we-need-better-testing-and-we-need-it-now/

 

Reducing Bartonella in Cats Utilizing Flea & Tick Collar

https://www.ncbi.nlm.nih.gov/pubmed/30709361

2019 Feb 1;12(1):69. doi: 10.1186/s13071-018-3257-y.

Effectiveness of a 10% imidacloprid/4.5% flumethrin polymer matrix collar in reducing the risk of Bartonella spp. infection in privately owned cats.

Abstract

BACKGROUND:

Bartonella henselae, Bartonella clarridgeiae and the rare Bartonella koehlerae are zoonotic pathogens, with cats being regarded as the main reservoir hosts. The spread of the infection among cats occurs mainly via fleas and specific preventive measures need to be implemented. The effectiveness of a 10% imidacloprid/4.5% flumethrin polymer matrix collar (Seresto®, Bayer Animal Health), registered to prevent flea and tick infestations, in reducing the risk of Bartonella spp. infection in privately owned cats, was assessed in a prospective longitudinal study.

METHODS:

In March-May 2015 [Day 0 (D0)], 204 privately-owned cats from the Aeolian Islands (Sicily) were collared (G1, n = 104) or left as controls (G2, n = 100). The bacteraemia of Bartonella spp. was assessed at enrolment (D0) and study closure (D360) by PCR and DNA sequencing both prior to and after an enrichment step, using Bartonella alpha proteobacteria growth medium (BAPGM).

RESULTS:

A total of 152 cats completed the study with 3 in G1 and 10 in G2 being positive for Bartonella spp. Bartonella henselae genotype I ZF1 (1.35%) and genotype II Fizz/Cal-1 (6.76%) as well as B. clarridgeiae (5.41%) were detected in cats of G2. Bartonella clarridgeiae was the only species detected in G1. Based on the yearly crude incidence of Bartonella spp. infection (i.e. 3.85% in G1 and 13.51% in G2; P = 0.03) the Seresto® collar achieved a preventative efficacy of 71.54%. The incidence of Bartonella spp. infection was more frequent in flea-infested cats (6/33, 18.18%) than in uninfested ones (7/112, 5.88%) (P = 0.036).

CONCLUSIONS:

Cats living in the Aeolian Islands are exposed to B. henselae and B. clarridgeiae. The Seresto® collar provided significant risk reduction against Bartonella spp. infection in outdoor cats under field conditions. Such a preventative tool could be a key contribution for decreasing the risk of Bartonella spp. infection in cats and thus ultimately to humans.

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**Comment**

Bartonella is a huge player with Lyme/MSIDS, and far more than cats & fleas are involved.  Many healthy people without cat exposure have contracted Bartonella:  

 https://madisonarealymesupportgroup.com/2019/01/23/chest-imaging-of-cat-scratch-disease-in-2-year-old-immunocompetent-baby-with-no-history-of-cat-contact/  Healthy 2 year old with no history of cat exposure.

https://madisonarealymesupportgroup.com/2018/07/10/bartonella-henselae-neuroretinitis-in-patients-without-cat-scratch  All the patients denied a history of a cat or any animal contact, or of having CSD findings.

https://madisonarealymesupportgroup.com/2018/07/05/cat-scratch-disease-in-a-1-5-year-old-girl-case-report/  Healthy 1.5-year-old girl who was seen in hospital for the sparing use of her left arm when crawling. Tested positively for Bartonella henselae.

https://madisonarealymesupportgroup.com/2018/04/03/encephalopathy-in-adult-with-cat-scratch-disease/  Case of a 53-year-old healthy man, presenting with confusion. Serology confirmed Bartonella henselae infection.

https://madisonarealymesupportgroup.com/2019/01/09/transverse-myelitis-guillain-barre-associated-with-bartonella/  Healthy 10 year old girl had coexisting transverse myelitis and Guillain-Barré syndrome (GBS) related to infection with Bartonella henselae.

https://madisonarealymesupportgroup.com/2018/11/05/skull-infection-due-to-bartonella/  While cats are implicated, this 3 year old had no significant medical history, presented at emergency department for a 2-week history of worsening scalp lump with redness.

https://madisonarealymesupportgroup.com/2018/03/04/bartonella-erythema-nodosum-atypical-presentations/  All immunocompetent hosts.

 

 

Anti-viral Therapy in Alzheimer’s- Clinical Trial

https://clinicaltrials.gov/ct2/show/NCT03282916

Anti-viral Therapy in Alzheimer’s Disease

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Know the risks and potential benefits of clinical studies and talk to your health care provider before participating. Read our disclaimer for details.
ClinicalTrials.gov Identifier: NCT03282916

Recruitment Status : Recruiting

First Posted : September 14, 2017
Last Update Posted : January 25, 2019
Sponsor:
Collaborators:
National Institutes of Health (NIH)
National Institute on Aging (NIA)
Information provided by (Responsible Party):
Davangere P. Devanand, New York State Psychiatric Institute
Study Description
Brief Summary:
Anti-viral therapy in Alzheimer’s disease will investigate the efficacy of treating patients with mild Alzheimer’s disease with the U.S.A marketed generic anti-viral drug Valtrex (valacyclovir, 500mg oral tablet). Valacyclovir at 2 g to 4 g per day, repurposed to treat Alzheimer’s disease, will be compared to matching placebo in the treatment of 130 mild AD patients (65 valacyclovir, 65 placebo) who test positive for herpes simplex virus-1 (HSV1) or HSV2. The study will be a randomized, double-blind, 18-month Phase II proof of concept trial.
Condition or disease Intervention/treatment Phase
Alzheimer DiseaseHerpes Simplex 1Herpes Simplex 2 Drug: ValacyclovirDrug: Placebo Phase 2
Detailed Description:

Many viruses are latent for decades before being reactivated in the brain by stress, immune compromise, or other factors. After the initial oral infection, herpes simplex virus-1 (HSV1) becomes latent in the trigeminal ganglion and can later enter the brain via retrograde axonal transport, often targeting the temporal lobes.

HSV1 can also enter the brain via olfactory neurons directly. HSV1 (oral herpes) and HSV2 (genital herpes) are known to trigger amyloid aggregation and their DNA is commonly found in amyloid plaques. Anti-HSV drugs reduce Aβ and p-tau accumulation in brains of infected mice. HSV1 reactivation is associated with tau hyperphosphorylation in mice and may play a role in tau propagation across neurons. In humans, recurrent reactivation with newly produced HSV1 particles, ‘drop by drop,’ may produce neuronal damage and eventually lead to neurodegeneration and Alzheimer’s disease (AD) pathology, partly due to effects on amyloid and tau. Clinical studies show cognitive impairment in HSV seropositive patients in different patient groups and in healthy adults, and antiviral treatments show robust efficacy against peripheral HSV infection. The study team will conduct the first-ever clinical trial to directly address the long-standing viral etiology hypothesis of AD which posits that viruses, particularly the very common HSV1 and HSV2, may be etiologic or contribute to the pathology of AD.

In patients with mild AD who test positive for serum antibodies to HSV1 or HSV2, the generic antiviral drug valacyclovir, repurposed as an anti-AD drug, will be compared at oral doses of 2 to 4 grams per day to matching placebo in the treatment of 130 patients (65 valacyclovir, 65 placebo) in a randomized, double-blind, 78-week Phase II proof of concept trial. Patients treated with valacyclovir are hypothesized to show smaller decline in cognition and functioning compared to placebo, and, using 18F-Florbetapir PET imaging, to show less amyloid accumulation than placebo over the 78-week trial. Through the use of tau PET imaging with the tracer 18F-MK-6240 at baseline and 78 weeks, patients treated with valacyclovir are hypothesized to show smaller increases in 18F-MK-6240 binding than patients treated with placebo from baseline to 78 weeks. Apolipoprotein E genotype at baseline, as well as changes in cortical thinning on structural MRI, olfactory identification deficits, and antiviral antibody titers from baseline to 78 weeks, will be evaluated in exploratory analyses. In patients who agree to lumbar puncture, plasma and CSF acyclovir will be assayed to establish the degree of CNS penetration of valacyclovir in mild AD, and the investigators will obtain CSF Aβ42, tau, p-tau for subset exploratory analyses with changes in outcome measures.

If this trial is successful, the investigators will apply for funding to conduct a larger, multicenter, Phase III study using a study design that will be informed by the results of this Phase II trial. This innovative Phase II proof of concept trial clearly has exceptionally high reward potential for the treatment of AD.

Study Design
Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 130 participants
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple (Participant, Care Provider, Investigator)
Primary Purpose: Treatment
Official Title: Anti-viral Therapy in Alzheimer’s Disease
Actual Study Start Date : February 12, 2018
Estimated Primary Completion Date : August 2022
Estimated Study Completion Date : August 2022
___________________
**Comment**
Remember, Alzheimer’s is a label, nothing more.  They are still trying to figure out what causes it.
Dr. Klinghardt has gone on record stating that he’s not had one MS, ALS, or Parkinson’s patient NOT test positive for Lyme:  https://madisonarealymesupportgroup.com/2019/01/23/never-had-a-single-ms-als-or-parkinsons-patient-in-past-5-years-who-didnt-test-positive-for-lyme-dr-klinghardt/

Growing Importance of Lone Star Ticks in a Lyme Disease Endemic County

The growing importance of lone star ticks in a Lyme disease endemic county: Passive tick surveillance in Monmouth County, NJ, 2006 – 2016

Robert A. Jordan ,Andrea Egizi

PLOS

Published: February 12, 2019  https://doi.org/10.1371/journal.pone.0211778

Abstract

As human cases of tick-borne disease continue to increase, there is a heightened imperative to collect data on human-tick encounters to inform disease prevention. Passive tick surveillance programs that encourage members of the public to submit ticks they have encountered can provide a relatively low-cost means of collecting such data. We report the results of 11 years of tick submissions (2006–2016) collected in Monmouth County, New Jersey, an Atlantic coastal county long endemic for Lyme disease. A total of 8,608 ticks acquired in 22 U.S. states were submitted, 89.7% of which were acquired in Monmouth County, from 52 of the County’s 53 municipalities. Seasonal submission rates reflected known phenology of common human-biting ticks, but annual submissions of both Amblyomma americanum and Dermacentor variabilis increased significantly over time while numbers of Ixodes scapularis remained static. By 2016, A. americanum had expanded northward in the county and now accounted for nearly half (48.1%) of submissions, far outpacing encounters with I. scapularis (28.2% of submissions). Across all tick species and stages the greatest number of ticks were removed from children (ages 0–9, 40.8%) and older adults (ages 50+, 23.8%) and these age groups were also more likely to submit partially or fully engorged ticks, suggesting increased risk of tick-borne disease transmission to these vulnerable age groups. Significantly more people (43.2%) reported acquiring ticks at their place of residence than in a park or natural area (17.9%). This pattern was more pronounced for residents over 60 years of age (72.7% acquired at home). Education that stresses frequent tick checks should target older age groups engaged in activity around the home. Our results strongly suggest that encounter rates with ticks other than I. scapularis are substantial and increasing and that their role in causing human illness should be carefully investigated.

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**Comment**

We’ve been told Amblyomma americium, .a.k.a Lone Star tick, only inhabits Southern states, and Dermacentor variabilis (Say), a.k.a. American dog tick or wood tick, is only found east of the Rocky Mountains, yet once again this proves the point ticks do not understand boundaries.  (Neither do birds, reptiles, deer, mice, fox, people, etc. – whom transport the ticks everywhere) 

The Lone Star tick is known to transmit Human Monocytic Ehrlichiosis, Tularemia, STARI, and is suspected of Lyme Disease and possibly Rocky Mountain Spotted Fever.  https://tickinfo.com/lonestartick

The CDC swears up and down it doesn’t transmit Lyme, yet, every advocate I know in the South says STARI looks, smells, and feels just like Lyme.  Patients in the South are tossed aside like yesterday’s garbage:  https://madisonarealymesupportgroup.com/2018/05/31/no-lyme-in-the-south-guess-again/

For the sordid history of the Southern Lyme/STARI fiasco, read here:  https://madisonarealymesupportgroup.com/2017/10/06/remembering-dr-masters-the-rebel-for-lyme-patients-who-took-on-the-cdc-single-handedly/  Excerpt:

Masters worked with Missouri entomologist, Dorothy Fier, who found borrelia in 2% of sampled lone star ticks and who supported Masters’ Missouri Lyme.  Despite publicity and validation, the CDC insisted that the EM rash was NOT diagnostic for LD for Missouri patients due to the fact that neither Ixodes dammini nor Ixodes pacificus were found there.

Masters worked with the CDC, who purposely tossed out data and manipulated the results.  Patients have been suffering ever since.

The wood tick is known to transmit Rocky Mountain spotted fever (RMSF), tularemia and can cause canine tick paralysis in dogs.  A high number of tularemia infected wood ticks have been found in Minnesota:  https://madisonarealymesupportgroup.com/2019/02/18/tularemia-in-minnesotan-ticks/  Across all sites, 128 (34%) of 378 pools were RT-PCR positive for F. tularensis.

Oops, that’s not supposed to happen….