https://www.ncbi.nlm.nih.gov/pubmed/31923195/

2020 Jan 10;15(1):e0227234. doi: 10.1371/journal.pone.0227234. eCollection 2020.

Molecular prevalence of Bartonella, Babesia, and hemotropic Mycoplasma species in dogs with hemangiosarcoma from across the United States.

Abstract

Hemangiosarcoma (HSA), a locally invasive and highly metastatic endothelial cell neoplasm, accounts for two-thirds of all cardiac and splenic neoplasms in dogs. Bartonella spp. infection has been reported in association with neoplastic and non-neoplastic vasoproliferative lesions in animals and humans. The objective of this study was to determine the prevalence of Bartonella spp. in conjunction with two other hemotropic pathogens, Babesia spp. and hemotropic Mycoplasma spp., in tissues and blood samples from 110 dogs with histopathologically diagnosed HSA from throughout the United States. This was a retrospective, observational study using clinical specimens from 110 dogs with HSA banked by the biospecimen repository of the Canine Comparative Oncology and Genomics Consortium. Samples provided for this study from each dog included: fresh frozen HSA tumor tissue (available from n = 100 of the 110 dogs), fresh frozen non-tumor tissue (n = 104), and whole blood and serum samples (n = 108 and 107 respectively). Blood and tissues were tested by qPCR for Bartonella, hemotropic Mycoplasma, and Babesia spp. DNA; serum was tested for Bartonella spp. antibodies.

  • Bartonella spp. DNA was amplified and sequenced from 73% of dogs with HSA (80/110)
  • hemotropic Mycoplasma spp. DNA was amplified from a significantly smaller proportion (5%, p<0.0001)
  • Babesia spp. DNA was not amplified from any dog

Of the 100 HSA tumor samples submitted,

  • 34% were Bartonella PCR positive (32% of splenic tumors, 57% of cardiac tumors, and 17% of other tumor locations)
  • Of 104 non-tumor tissues, 63% were Bartonella PCR positive (56% of spleen samples, 93% of cardiac samples, and 63% of skin/subcutaneous samples).
  • Of dogs with Bartonella positive HSA tumor, 76% were also positive in non-tumor tissue.
  • Bartonella spp. DNA was not PCR amplified from whole blood.

This study documented a high prevalence of Bartonella spp. DNA in dogs with HSA from geographically diverse regions of the United States. While 73% of all tissue samples from these dogs were PCR positive for Bartonella DNA, none of the blood samples were, indicating that

whole blood samples do not reflect tissue presence of this pathogen.

Future studies are needed to further investigate the role of Bartonella spp. in the development of HSA.

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**Comment**

And here, we see exactly what patience experience in reality: negative blood tests but positive tissue samples.  Dr. Ericson has found Bartonella in tissues directly by where a PICC line was removed:  https://madisonarealymesupportgroup.com/2019/02/27/advanced-imaging-found-bartonella-around-pic-line/

This is true not only for Bartonella but for Lyme as well as all of the coinfections.  Doctors that rely only on testing are missing patients right and left.

Please spread the word.